He was reluctant, and after six months was brought before the Emperor again and again his request was made. Once brought to him, the Emperor requested that he resurrect Soul Edge to defeat the Ottoman Sultan Barbaros, who wielded Soul Calibur. Siegfried is saved from the Evil Seed by a group sent by the Masked Emperor to investigate it. Siegfried accepted and carried the blade into many slaughters, until he finally succumbed to the sword's restored powers, transforming him into the Azure Knight, Nightmare. At that moment Siegfried heard the voice of the wicked sword, who arranged a deal with him: Siegfried would help the blade to restore itself by gathering souls, and Soul Edge would resurrect his father Frederick. Siegfried raised his zweihänder and defeated the fiery Inferno, breaking his sword in the process, earning the right to wield the Soul Edge for himself.Īt the precise moment that Siegfried obtained the cursed blade, the Evil Seed emanated from him in a beam of light that rained across the entire world, leading only to death and destruction. But as he reached for it, the corpse lit up in a hellish blaze of fire and attacked him. He finally managed to come across the legendary weapon Soul Edge, lying beside the defeated corpse of the dead pirate, Cervantes de Leon. Frustrated, he continued to search for weeks for the Soul Edge, growing increasingly more insane for its power as time passed. During a siege on the castle, Siegfried saw an opportunity to take Sir Stefan's prized possession, the sword Grimblade, and killed him, running off with it into the Black Woods nearby the castle but the sword held no power. He traveled first to Ostrheinsburg Castle, the refuge of the noble Sir Stefan, who he believed to be the possessor of the Soul Edge and he joined in its forces as a Landsknecht, rising in its ranks with the hopes of acquiring Stefan's self-proclaimed legendary blade. Hearing rumors of the invincible Soul Edge, Siegfried came to believe his father's killer could only be killed with said weapon. In mere seconds, Siegfried's mind had broken upon which point he ran straight into the forest, becoming more and more insane as he ruminated over his previous acts, until he came to a point at which he psychologically convinced himself that his father's murderer was actually someone other than himself. Siegfried even managed to kill the commander himself but as he held the commander's severed head in triumph, the moonlight shone on its face, and Siegfried screamed as he realized he held the head of his own father. That night, the " Schwarzwind" ambushed a group of war-weary soldiers, too tired to retaliate. Over the course of the next ten to fifteen years, Frederick spent a lot of his time teaching his son swordsmanship.Įmbarking on a foreign crusade, Frederick promised Margaret that he would return unfortunately, without his father's guidance, Siegfried began to socialize with a delinquent clan of teenagers, and he soon became the leader of their band of thieves known as " Schwarzwind," meaning "black wind." In a misguided act of national pride, the band devised a plan to attack the knights who ran away from the crusades, as they were bound to carry valuable possessions, and were too cowardly to deserve respect in any case. When Frederick returned after nine months of battle, he rejoined Margaret, having given birth to a son, whom he named Siegfried, after the famous hero. One of the champions of the people, a brave knight named Frederick, met and fell in love with a woman named Margaret while on campaign and they spent the night together. With its forces weakened by the Italian Wars, the German peasants finally had a fighting chance. 5.5 Soulcalibur IV/Soulcalibur: Broken Destinyĭuring the late sixteenth century, the oppressed peasants of Germany were revolting against the Holy Roman Empire.5.4 Soulcalibur III/Soulcalibur Legends.
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YouTube, as the world's second-largest search engine, reigns supreme in the realm of online video platforms. Free download this free YouTube MP3 converter on Mac. The best free YouTube to MP3 converters are a great way to enjoy your favorite audio content from YouTube whenever and wherever you want - at no cost. Nice logo, nice menus, user-friendly, worked first time out of the box just like mac stuff is supposed to Very impressed. Downloading videos and music from YouTube has never been easier and faster with the helper installation. Guys, your yt to mp3 converter for mac osx absolutely rips So nice to find a well designed program that works as its supposed to, without any hassles and timewasting like so many others Ive found. Respondents could also have protected themselves from erroneous on-the-scene testing by electing to submit to urine or blood tests, see n. Finally, as to operator error, the defendant retains the right to cross-examine the law enforcement officer who administered the Intoxilyzer test, and to attempt to raise doubts in the mind of the factfinder whether the test was properly administered. For defendants whose test results might have been affected by either of these factors, it remains possible to introduce at trial evidence demonstrating that the defendant was dieting at the time of the test or that the test was conducted near a source of radio waves. As to improper measurements, the parties have identified only two sources capable of interfering with test results: radio waves and chemicals that appear in the blood of those who are dieting. Respondents could have utilized these data to impeach the machine’s reliability. To protect against faulty calibration, California gives drunken driving defendants the opportunity to inspect the machine used to test their breath as well as that machine’s weekly calibration results and the breath samples used in the calibrations. Respondents were perfectly capable of raising these issues without resort to preserved breath samples. See Brief for Respondents 32-34 Brief for California Public Defender’s Association et al. Respondents and amici have identified only a limited number of ways in which an Intoxilyzer might malfunction: faulty calibration, extraneous interference with machine measurements, and operator error. “Even if one were to assume that the Intoxilyzer results in this case were inaccurate and that breath samples might therefore have been exculpatory, it does not follow that respondents were without alternative means of demonstrating their innocence. However, in so doing, the Trombetta Court’s ruling proceeded on the assumption that as a general rule, the defendant would still be allowed a fair opportunity to rebut the test results at trial: California, the court held that the failure to preserve a breath sample that was capable of being captured, so that an independent analysis could be performed, did not violate a Defendant’s right to present a defense. The right to confront evidence – machine or inspector or operator or source code The right to be free from governmental interference with defense witnessesĥ. The right to present expert testimony (indigency)Ĥ. The right of compulsory process – subpoenasģ. The right to present a meaningful defense and meaningful evidenceĢ. In all criminal prosecutions, the accused shall enjoy the right to a speedy and public trial, by an impartial jury of the State and district wherein the crime shall have been committed, which district shall have been previously ascertained by law, and to be informed of the nature and cause of the accusation to be confronted with the witnesses against him to have compulsory process for obtaining witnesses in his favor, and to have the Assistance of Counsel for his defense.ġ. The Sixth Amendment to the United States Constitution states: In the light of present day scientific advances, due process, it seems to us, requires a second sample. Until we are prepared to accept both, we take the view that when the State conditionally requires that a person submit to a breath, blood, or urine test conducted by the State, fundamental fairness requires that an additional sample be available to the person tested for his or her own independent analysis. “e do not believe in the infallibility of human agencies, any more than we believe in infallible technology. People v Orth, 124 Ill 2d 326, 530 NE2d 210 (1988)ĭiscovering What YOU Need to Win (6 th Amendment) It is an “empirical fact that breathalyzer tests, while generally valid, are not fool-proof.” National College for DUI Defense 2009 “Discovering What You Need to Win” Some of our favorite building activities include: Littles love to build - not to mention it’s a great opportunity for independent play.
Ithaca Kitty started a craze for plush toys that lasted from 1892 until after WWI.Later, the company used Chessie and her kittens, Nip and Tuck, to promote war bonds for World War II. Chessie, a tabby cat, was featured in the famous “Sleep like a Kitten” ad campaign for the Chesapeake and Ohio Railway (C&O), to promote the company’s sleeper cars in 1933.Tabby cat Stubbs, the mayor of Talkeetna, Alaska, was elected in 1997 and served for 20 years until he passed away.The Cheshire Cat in Lewis Carroll’s Alice’s Adventures in Wonderland is a tabby cat.Cartoon characters Heathcliff, Garfield, and Bill the Cat (from Bloom County) are all tabbies.Milo, from the 1989 film, The Adventures of Milo and Otis, was an orange tabby.Tabby Morris the Cat became the mascot for 9 Lives cat food in 1969.We know that people began referring to striped cats as “tabi” cats as early as the 1690s. The stripes on tabby cats apparently reminded the English people of this special cloth. Interestingly, the French word for “striped silk taffeta” is “tabis.” English people called the cloth “tabby,” short for Attabiya. The silk cloth produced by this district eventually found its way to England, and became very popular. The district itself was named for Attab ibn Asid, who was the governor of Mecca in the early 600s. There is a district in Baghdad called Attabiya, that was known for making beautiful striped silk cloth. The word “tabby” comes from the Muslim world, and a special silk fabric. There are many, many examples of this strategy in nature. The American Bittern, for example, which is a vertically striped bird, stretches its neck up and sways when approached by a predator, looking for all the world just like the reeds in which it lives. The trick isn’t unique to cats, by the way. In fact, this non-agouti gene doesn’t always work perfectly, which is why many solid-colored cats have a hint of striping. The non-agouti gene prevents the hair from producing that lighter pigment while the strand is growing. It’s a gene that just hides the tabby-ness. The non-agouti gene isn’t a “non-tabby” gene. Here’s where you might argue that a solid-colored cat is, in fact, not a tabby cat. In other words, there will be no gene to stop and start hair pigmentation and he will end up looking like a solid-colored cat. But if a cat gets two recessive genes – one from his mom and one from his dad – he will be non-agouti. The agouti gene is dominant, which means if a cat gets even one from a parent, he will have agouti fur and look like a tabby cat. That's how agouti hair strands get their bands of color. Then it slows the pigmentation down in the middle, and then speeds it up again at the end. When a hair is first growing, the agouti gene allows it to be fully pigmented. The agouti gene messes with pigmentation while each individual strand of hair is growing. The first set of genes that we need to talk about when discussing tabby-ness is the agouti gene, which allows hairs to be banded with darker and lighter colors. Western Blots Are Semi-Quantitativeīefore we take you through the necessary steps for quantifying your western blots, we need to clarify that western blots are only semi-quantitative. This means you can see how the levels of your protein of interest change between time points, conditions, and treatments. The value you obtain is directly proportional to the concentration of your protein of interest.Ī quantitative western blot will allow you to measure relative changes between different conditions, so rather than just saying a protein is present or absent in a sample, you can go further and say your target protein is present x-times higher in one sample compared to another for example. Why Should You Perform Western Blot Quantification?įirst, what do we actually mean by western blot quantification? Put simply, this refers to the measurement of the signal emitted by your target protein. While it can be used to simply show the presence or absence of a protein in a sample, it can tell you so much more if you take the next step and perform western blot quantification.Īs scientists, we love nothing more than quantitative data! Here, we have pulled together the 4 key steps for you to perform western blot quantification. It separates proteins based on size and then uses antibodies to detect specific proteins within the sample. Western blotting is a commonly used lab technique to identify proteins within a sample. Fortunately, this Duplicate File Finder is a tool that has been trusted by thousands of users worldwide to simplify this process. Whether you're a casual computer user or a seasoned IT professional, dealing with duplicate files can be a frustrating experience. 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This powerful tool lets you easily sort your files based on their types and quickly eliminate duplicates for free. Fortunately, iBeesoft Duplicate File Finder makes this task much easier and quicker. It can take hours to review each file and determine which are necessary and which can be removed. Sorting out files can be a tedious and time-consuming task, especially when you're dealing with duplicates. Quickly and Safely Remove Your Duplicate Files But we also incorporate physical elements into it using the 3 Senese with your 3 words tips below. The majority of the boards we create are digital. We discussed in Part 1 of our series all about mood boards and in Part 2we provided YES or NO questions to help you decide which style of board is right for you. The next step is how you will display everything together. Artist and Illustrator Mood Board Collection Artist and Illustrator Mood Board As long as it passes at least one, then add it to the board. They can include one or all three (the choice is yours). Our Tagline: Fun and bold patterns that bring your products to lifeīefore you add anything to the board, use these three words for picking every font, colour or photo. TaglineĪs we have discussed throughout this series, a tagline is a short line of copy that sums up everything the Mood Board represents.Īs we do with all our Mood Boards, we like to think of three words that best sum up the board. And this is exactly what we wanted as we also used it for the name of our website template. Being short always makes it easier to refer to it or for people to remember it. It felt like a short, sweet and catchy name. We choose Milla as the name of our Mood Board. Our Website Template Theme: Artists, Illustrators & Surface Pattern Designer Mood Board Give your Mood Board a Name You know what you need to wear, what it could involve, and the overall look and feel of the night.Ī theme gives you direction and a path to follow and, that’s why it’s so important to have one for your mood board. (My favourite type of party!) Now you know what to expect. It’s similar if you receive an invitation to a party. We love using a theme in everything we create. How could they incorporate their work and the look and feel of their branding into their website? We knew exactly what direction to take and, here’s how we did it. – Heading & Body Copy Fonts Artist and Illustrator Mood BoardĪfter listening and talking to numerous creatives, the same issue kept coming up. We love using mood boards as inspiration, and we hope that something within our boards will spark inspiration within you. We not only included one but five mood boards. This fun and vibrant Milla mood board set would have to be our favourite one we have created. Pin your favourite or pin them all! Artist and Illustrator Mood Board – Collection Artist & Illustrators Modd Boards (this is the blog you’re reading)Ĭheck out the Artist and Illustrator Mood Board Collection above.Destination Wedding Photographer Mood Boards.Our Favourite 5-step Process to Creating a Mood Board.All these steps help form a mood board that not only inspires but results in a design that captures exactly what your client was after.īefore we get started, missed a part of our Mood Board Series? Catch up below. You’ll discover our exact process from picking images to choosing a colour palette, font pairing and everything in-between. We are back for Part 4 of our Inspirational Mood Board Series – Artists and Illustrator Edition. And, the perfect place to start is with our Artist and Illustrator Mood Board. We want to take you behind the scenes leading up to the launch of our latest Showit Website Template – Milla. "I was lying there in bed, thinking to myself: this is exactly how I feel," she says. Termini recalls the story sticking with her after she left the movie theater. In the years since, it's one of the few movies she's rewatched over a dozen times. Erin often hears stories of other trans people today that went through very similar journeys as teenagers, even frequenting the same sites that she visited.Įven for some folks who didn't realize they were transgender at the time, the movie stuck with them for reasons they couldn't fully explain.Ĭalista Termini watched The Matrix when it first released in theaters. Many trans people in that generation turned to the internet for those reasons. That knowledge in and of itself was enough for me to be like, yes, this is what I want. "But on the internet, it was like this is possible, and nothing could stop me from knowing it was possible. "There was no way that I was ever going to learn about that from my parents or from school or from any resources that would have ever been given to me," she recalls. She remembers finding message boards targeted towards trans people, offering early glimpses into gender-affirming medical care. Log on and one can pick from a glut of increasingly-specific identity tests, or find a community of like-minded people for any niche interest.Ĭarrie-Anne Moss and Keanu Reeves in The Matrix Nowadays, the internet is inextricably meshed with our lives to a degree where we might take its self-discovery possibilities for granted. The character ended up presenting as a woman in both dimensions.īut crucially, it's the spirit of self-actualization that resonates so strongly with many trans people – the existence of a true identity beyond your physical form, an ideal self that you can seize control of via the internet from an uncaring system. "I think the studio wasn't ready for that," he said. In an interview with Entertainment Weekly, Keanu Reeves, who played Neo in the movies, recalled an early draft of the script that was later changed. The red pill that Neo ends up taking is similar to what estrogen pills, used for hormone replacement therapy, looked like in the nineties.Īnother example: the Wachowskis wanted the character Switch to present as male in the real world while presenting as female in the Matrix, as an explicit nod to the idea of transness in an online world. The blue pill will allow him to continue in ignorance. The red pill will open his eyes to the truth that he is living in a simulation. For example, Neo is offered a choice between a red and blue pill. She added that she didn't know how present her transness was in her mind as they were writing the movie, but that much of the desire for transformation in the films stemmed from her closeted point of view at the time.īut long before this was confirmed, many of their trans fans were latching onto the movie anyways.Ī couple of the more overt parallels have garnered attention over the years. "I'm glad that it has gotten out that, you know, that was the original intention, but the world wasn't quite ready – the corporate world wasn't ready for it." A highly anticipated and unexpected fourth sequel, The Matrix Resurrections, is out this week.īut beyond its legacy in pop culture, The Matrix has long held a special place in the hearts of many trans people for the way it so perfectly encapsulates many essential facets of their identities. In the approximately two decades following the release of the first movie in 1999, The Matrix has endured in pop culture for its portrayal of internet culture, philosophical themes, revolutionary special effects, and slick leather-clad fashion – just to name a few. He has always questioned his reality, but now he must choose whether or not to embrace his role in humanity's battle to destroy the illusion. The Matrix follows computer hacker Neo, who discovers the shocking truth that the life he knows is really a complex computer simulation designed by intelligent machines to enslave humanity. This was back when you had to compete with a landline to load a web page, and her mom eventually bought a separate phone line because she was online so often. When she got back from school, the first thing she would do was run to her computer, where she was allowed to be herself again. At the time, Reed was just beginning to understand that she was transgender. 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